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"Twilight" was, at the time of its release, not particularly popular in the United States or the UK. However, the song gained cult status within Japan when the song was used in a short film known as DAICON Film or DAICON IV. The animated short was shown at the 1983 Nihon SF Taikai convention.
Here is a remastered version of the DAICON IV animation, as well as it prequel, DAICON III:
Many a nerd and nerdess have spent hours trying to identify the countless sci-fi and fantasy franchises that appear in DAICON IV. There was a Lasedisk containing both DAICON films, but due to copyright issues sorrowing the unauthorized used of "Twlight," it is doubtful this Laserdisk will ever again see the light of day. Alas, all we have are old transfers of the original 8mm films.
DAICON IV was produced by a group of amateur animators who would later go on to found the Gainax animation studio... producer of such awesome shows as FLCL and Tengen Toppa Gurren Lagann, as well as some other emo giant robot show that only dorks and emos like.
"Twilight" was again used in 2005 as the theme for the Japanese Drama "Densha Otoko" (Train Man). (The show itself is based on the Japanese urban legend of the Train Man.) Contrary to popular rumor, the animated opening sequence was made by the anime studio Gonzo, not Gainax. But, during a magazine interview, the producers at Gonzo did recognize the original short and thank its creators.