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Enzyme Kinetics/Biochemistry help.
Johannen
Registered User regular
Registered User regular
Right, I have a graph of p-nitrophenol produced (in micromol) against time and i've gained the rate of the enzyme-catalyzed reaction from the initial slope of the curve. (I calculated change in Y/change in X to get the slope and therefore the rate) (turned out to be 0.56mmol/min)
I'm stuck as hell on how to find out the number of enzyme units in each mL of partially purified phosphotase from the rate that I got.
I know how to calculate the specific activity because i've got the protein determination showing that it contained 20.9mg/mL-1, so I just need to divide my number of enzyme units per mL by 20.9mg/mL-1.
The preparation was assayed by diluting the solution 1:100 and 5mL of the diluted solution and the amount of p-nitrophenol produced being recorded over 10 minutes.
Anyone here know what to do, or where I could go to get an answer for this question?
I'm stuck as hell on how to find out the number of enzyme units in each mL of partially purified phosphotase from the rate that I got.
I know how to calculate the specific activity because i've got the protein determination showing that it contained 20.9mg/mL-1, so I just need to divide my number of enzyme units per mL by 20.9mg/mL-1.
The preparation was assayed by diluting the solution 1:100 and 5mL of the diluted solution and the amount of p-nitrophenol produced being recorded over 10 minutes.
Anyone here know what to do, or where I could go to get an answer for this question?
Johannen on
0